| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Rothia aeria | GGAAACCCCTGCTCTGAACA | CTGCGCACACGAGCTTTT | 59.89 | 59.37 | 171 | 102 |
100.00%
|
100.00%
|
| Primer 52 | Rothia aeria | CCTGGGTCACCGCTGATTT | ACGCAAATCCCAGGTGCT | 60.00 | 59.56 | 293 | 102 |
100.00%
|
100.00%
|
| Primer 53 | Rothia aeria | TGGGTGACTGCGGTGATTC | TGCCGAGCCAACCAGTTT | 60.00 | 59.81 | 281 | 102 |
100.00%
|
100.00%
|
| Primer 54 | Rothia aeria | AGTACGGTGTGATGGCTGC | TGCCGAGCCAACCAGTTT | 60.08 | 59.81 | 217 | 102 |
100.00%
|
100.00%
|
| Primer 55 | Rothia aeria | GGTGTGATGGCTGCGAGTA | TGCCGAGCCAACCAGTTT | 59.78 | 59.81 | 212 | 102 |
100.00%
|
100.00%
|
| Primer 56 | Rothia aeria | ACGGGTTGGCGTACGTTT | CACCAGCACCTCCAAGACA | 59.89 | 59.55 | 224 | 102 |
100.00%
|
100.00%
|
| Primer 57 | Rothia aeria | ACGGGTTGGCGTACGTTT | CGCCACCGAGAAAATAGCG | 59.89 | 59.36 | 287 | 102 |
100.00%
|
100.00%
|
| Primer 58 | Rothia aeria | ATGCCCAGCCGACGAAAA | TTCGGTGGTTGCGAGCAT | 59.97 | 59.97 | 172 | 101 |
100.00%
|
100.00%
|
| Primer 59 | Rothia aeria | ACGAAGGTCGCGTCATCA | TGAGCGAATGCAGCAGGT | 59.04 | 59.65 | 273 | 101 |
100.00%
|
100.00%
|
| Primer 60 | Rothia aeria | TGGCCAGCGGTAACACAT | TGGCCTTGAGCAGCGAAA | 59.25 | 59.89 | 283 | 101 |
100.00%
|
100.00%
|